A
PROFILE
UPM's Staff Profile
YBHG. PROFESOR DR. TAN WEN SIANG
PROFESSOR
FACULTY OF BIOTECHNOLOGY AND BIOMOLECULAR SCIENCES
wstan@upm.edu.my
UPM WEBSITE
PROFILE
wstan
BM
EN

PROFESSIONAL

NAME :
(Ybhg. Profesor Dr.) TAN WEN SIANG
POST :
PROFESSOR
ENTITY :
FACULTY OF BIOTECHNOLOGY AND BIOMOLECULAR SCIENCES

FIELD OF SUPERVISION
MEDICAL BIOTECHNOLOGY / MICROBIOLOGY / MOLECULAR BIOLOGY AND GENETIC ENGINEERING / NANOBIOTECHNOLOGY / VIROLOGY
EDUCATION
PHD
UNIVERSITY OF EDINBURGH, SCOTLAND, UNITED KINGDOM
Graduated : 1997
MASTER SAINS
UNIVERSITI PERTANIAN MALAYSIA, SERDANG, SELANGOR
Graduated : 1994
BACELOR SAINS
UNIVERSITI PUTRA MALAYSIA, SERDANG, SELANGOR
Graduated : 2006
BOOK PUBLICATION
1. A Laboratory Manual on Molecular Techniques For Identification of Bacteria
    ISPN: 978-983-44346-0-1
JOURNAL PUBLICATION
1. Purification of bacteriophage M13 by anion exchange chromatography
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
2. Nanoglue: An alternative way to display cell-internalizing peptide at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
3. Solution structure and in silico binding of a cyclic peptide with hepatitis B surface antigen
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
4. Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
5. Biochemical and molecular identification of Enterococcus spp. from red pitaya
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
6. Phage display creates innovative applications to combat hepatitis B virus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
7. RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
8. Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
9. Negative chromatography of hepatitis B virus-like particle: comparative study of different adsorbent designs
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
10. Virus-like Particle of Macrobrachium rosenbergii Nodavirus Produced in Spodoptera frugiperda (Sf9) Cells is Distinctive from that Produced in Escherichia coli
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
11. Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
12. Production of long helical capsid of Nipah virus by Pichia pastoris
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
13. Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
14. Enhancement of the yield of long helical structure of recombinant nucleocapsid protein of Newcastle disease virus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
15. pH-responsive Virus-like Nanoparticles with Enhanced Tumour-targeting Ligands for Cancer Drug Delivery
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
16. Tracking virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
17. Selection of high affinity ligands to Hepatitis B core particles from a phage-displayed cyclic peptide library
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
18. Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
19. Purification of rabbit polyclonal immunoglobulin G with ammonium sulphate precipitation and mixed-mode chromatography
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
20. A versatile and economical method for the release of recombinant proteins from Escherichia coli by 1-propanol cell disruption
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
21. Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
22. Delivery of chimeric hepatitis B core particles into liver cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
23. Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
24. Mechanical cell disruption of Escherichia coli for the release of recombinant green fluorescent protein
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
25. Oligomerization state of the multimerization domain of Nipah virus phosphoprotein
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
26. Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7
    Journal type: Citation-Indexed - Scopus
27. A Simple Add-and-Display Method for Immobilisation of Cancer Drug on His-tagged Virus-like Nanoparticles for Controlled Drug Delivery
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
28. Isolation and identification of lactic acid bacteria from fermented red dragon fruit juices
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
29. A phage-displayed cyclic peptide that interacts tightly with the immunodominant region of hepatitis B surface antigen
    Journal type: Citation-Indexed - Scopus
30. Mutagenesis of the Nucleocapsid Protein of Nipah Virus Involved in Capsid Assembly
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
31. N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
32. An N-terminal extension to the hepatitis B virus core protein forms a poorly ordered trimeric spike in assembled virus-like particles
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
33. Expression and purification of the matrix protein of Nipah virus in baculovirus-insect cell system
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
34. Production of the matrix protein of Nipah virus in Escherichia coli: virus-like particles and possible application in diagnosis
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
35. Display of Hepatitis B Virus PreS1 Peptide on bacteriophage T7 and Its Potential in Gene Delivery into HepG2 Cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
36. Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
37. A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
38. Hepatitis B virus peptide inhibitors: solution structures and interactions with the viral capsid
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
39. Negative chromatography purification of hepatitis B virus-like particles using poly(oligo(ethylene glycol) methacrylate) grafted cationic adsorbent
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
40. Production of the virus-like particles of Nipah virus matrix protein in Pichia pastoris as diagnostic reagents
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
41. Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
42. Purification of recombinant hepatitis B core antigen from unclarified Escherichia coli feedstock using phage-immobilized expanded bed adsorption chromatography
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
43. Statistical optimization of green fluorescent protein production from Escherichia coli BL21 (DE3)
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
44. Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
45. A phage-displayed single chain variable fragment that interacts with hepatitis B core antigen: library construction, selection and diagnosis
    Journal type: Citation-Indexed - Scopus
46. Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
47. Advances in the study of nodavirus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
48. A dual bioconjugated virus-like nanoparticle as a drug delivery system and comparison with a pH-responsive delivery system
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
49. Targeted Delivery of Cell Penetrating Peptide Virus-like Nanoparticles to Skin Cancer Cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
50. A 12-residue epitope displayed on phage T7 reacts strongly with antibodies against foot-and-mouth disease virus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
51. Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
52. Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
53. Peptide inhibitors of Macrobrachium rosenbergii nodavirus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
54. Efficacy and potential of phage therapy against multidrug resistant Shigella spp.
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
55. Thermally-responsive Virus-like Particle for Targeted Delivery of Cancer Drug
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
56. Infectious hematopoietic necrosis virus: advances in diagnosis and vaccine development
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
57. Shielding of Hepatitis B Virus-Like Nanoparticle with Poly(2-ethyl-2-oxazoline)
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
58. Recent Advances in the Vaccine Development against Middle East Respiratory Syndrome-coronavirus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
59. Stealth Coating of Nanoparticles in Drug Delivery System
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
60. Immunological Analysis of the Hepatitis B Virus ‘a’ Determinant Displayed on Chimeric Virus-like Particles of Macrobrachium rosenbergii Nodavirus Capsid Protein Produced in Sf9 Cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
61. Advances in the Diagnosis of Foot-and-Mouth Disease
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
62. Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
63. Targeted Delivery of 5-Fluorouracil-1-Acetic Acid (5-FA) to Cancer Cells Overexpressing Epithelial Growth Factor Receptor (EGFR) using Virus-Like Nanoparticles
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
64. Chimeric Virus-like Particles of Prawn Nodavirus Displaying Hepatitis B Virus Immunodominant Region: Biophysical Properties and Cytokine Response
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
65. Hepatitis B virus-like particle: targeted delivery of plasmid expressing short hairpin RNA for silencing the Bcl-2 gene in cervical cancer cells
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
66. Regulation of proteolytic activity to improve the recovery of Macrobrachium rosenbergii nodavirus capsid protein
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
67. The Macrobrachium rosenbergii nodavirus: a detailed review of structure, infectivity, host immunity, diagnosis and prevention
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
68. Challenges and Complications of Poly(lactic-co-glycolic acid)-Based Long-Acting Drug Product Development
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
69. Quartz crystal microbalance-based biosensing of hepatitis B antigen using a molecularly imprinted polydopamine film
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
70. Virus-like particles of Macrobrachium rosenbergii nodavirus: Particle size and capsid protein assembly domain
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
71. Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
72. Meta-analysis of studies on protection provided by different prophylactic agents, their routes of administration and incubation times against nodavirus infection in Macrobrachium rosenbergii
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
73. Next-generation viral nanoparticles for targeted delivery of therapeutics: Fundamentals, methods, biomedical applications, and challenges
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
74. Broadly cross-reactive immune responses in chickens immunized with chimeric virus-like particles of nodavirus displaying the M2e originated from avian and human influenza A viruses
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
75. Chimeric virus-like particles of nodavirus displaying M2e of human and avian influenza A viruses as a potential dual-use vaccine: Inducing a broader immune response and protecting mice against viral infections
    Journal type: Citation-Indexed Journal - Science Citation Index (JCR)
PUBLICATION
2025
Proteomic profiling of the Macrobrachium rosenbergii nodavirus infection: A study of early to late-stage infection in vitro
2025
Broadly cross-reactive immune responses in chickens immunized with chimeric virus-like particles of nodavirus displaying the M2e originated from avian and human influenza A viruses
2025
Chimeric virus-like particles of nodavirus displaying M2e of human and avian influenza A viruses as a potential dual-use vaccine: Inducing a broader immune response and protecting mice against viral infections
2023
Meta-analysis of studies on protection provided by different prophylactic agents, their routes of administration and incubation times against nodavirus infection in Macrobrachium rosenbergii
2023
Next-generation viral nanoparticles for targeted delivery of therapeutics: Fundamentals, methods, biomedical applications, and challenges
2023
Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus
2022
Challenges and Complications of Poly(lactic-co-glycolic acid)-Based Long-Acting Drug Product Development
2022
Virus-like particles of Macrobrachium rosenbergii nodavirus: Particle size and capsid protein assembly domain
2022
Quartz crystal microbalance-based biosensing of hepatitis B antigen using a molecularly imprinted polydopamine film
2021
Hepatitis B virus-like particle: targeted delivery of plasmid expressing short hairpin RNA for silencing the Bcl-2 gene in cervical cancer cells
2021
Chimeric Virus-like Particles of Prawn Nodavirus Displaying Hepatitis B Virus Immunodominant Region: Biophysical Properties and Cytokine Response
2021
Regulation of proteolytic activity to improve the recovery of Macrobrachium rosenbergii nodavirus capsid protein
2021
The Macrobrachium rosenbergii nodavirus: a detailed review of structure, infectivity, host immunity, diagnosis and prevention
2020
Stealth Coating of Nanoparticles in Drug Delivery System
2020
Targeted Delivery of 5-Fluorouracil-1-Acetic Acid (5-FA) to Cancer Cells Overexpressing Epithelial Growth Factor Receptor (EGFR) using Virus-Like Nanoparticles
2020
Advances in the Diagnosis of Foot-and-Mouth Disease
2020
Immunological Analysis of the Hepatitis B Virus ‘a’ Determinant Displayed on Chimeric Virus-like Particles of Macrobrachium rosenbergii Nodavirus Capsid Protein Produced in Sf9 Cells
2020
Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases
2019
Thermally-responsive Virus-like Particle for Targeted Delivery of Cancer Drug
2019
Infectious hematopoietic necrosis virus: advances in diagnosis and vaccine development
2019
Recent Advances in the Vaccine Development against Middle East Respiratory Syndrome-coronavirus
2019
Efficacy and potential of phage therapy against multidrug resistant Shigella spp.
2019
Shielding of Hepatitis B Virus-Like Nanoparticle with Poly(2-ethyl-2-oxazoline)
2018
A 12-residue epitope displayed on phage T7 reacts strongly with antibodies against foot-and-mouth disease virus
2018
Peptide inhibitors of Macrobrachium rosenbergii nodavirus
2018
A dual bioconjugated virus-like nanoparticle as a drug delivery system and comparison with a pH-responsive delivery system
2018
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture
2018
Targeted Delivery of Cell Penetrating Peptide Virus-like Nanoparticles to Skin Cancer Cells
2018
Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems
2017
Biosensing of hepatitis B antigen with poly(acrylic acid) hydrogel immobilized with antigens and antibodies
2017
Advances in the study of nodavirus
2017
Tracking virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells
2017
Virus-like Particle of Macrobrachium rosenbergii Nodavirus Produced in Spodoptera frugiperda (Sf9) Cells is Distinctive from that Produced in Escherichia coli
2017
A Simple Add-and-Display Method for Immobilisation of Cancer Drug on His-tagged Virus-like Nanoparticles for Controlled Drug Delivery
2016
A versatile and economical method for the release of recombinant proteins from Escherichia coli by 1-propanol cell disruption
2016
Negative chromatography of hepatitis B virus-like particle: comparative study of different adsorbent designs
2016
Expression and purification of the matrix protein of Nipah virus in baculovirus-insect cell system
2016
pH-responsive Virus-like Nanoparticles with Enhanced Tumour-targeting Ligands for Cancer Drug Delivery
2016
Production of the virus-like particles of Nipah virus matrix protein in Pichia pastoris as diagnostic reagents
2015
Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
2015
Purification of rabbit polyclonal immunoglobulin G with ammonium sulphate precipitation and mixed-mode chromatography
2015
Induction of Humoral and Cell-Mediated Immune Responses by Hepatitis B Virus Epitope Displayed on the Virus-Like Particles of Prawn Nodavirus
2015
Negative chromatography purification of hepatitis B virus-like particles using poly(oligo(ethylene glycol) methacrylate) grafted cationic adsorbent
2015
An N-terminal extension to the hepatitis B virus core protein forms a poorly ordered trimeric spike in assembled virus-like particles
2015
Hepatitis B virus peptide inhibitors: solution structures and interactions with the viral capsid
2014
A potential universal vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
2014
A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method
2014
RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein
2014
Phage display creates innovative applications to combat hepatitis B virus
2014
Biochemical and molecular identification of Enterococcus spp. from red pitaya
2013
Enhancement of the yield of long helical structure of recombinant nucleocapsid protein of Newcastle disease virus
2013
Solution structure and in silico binding of a cyclic peptide with hepatitis B surface antigen
2013
Display of the VP1 epitope of foot-and-mouth disease virus on bacteriophage T7 and its application in diagnosis
2013
Oligomerization state of the multimerization domain of Nipah virus phosphoprotein
2013
Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR
2013
Purification of long helical capsid of Newcastle disease virus from Escherichia coli using anion exchange chromatography
2012
Statistical optimization of green fluorescent protein production from Escherichia coli BL21 (DE3)
2012
Mechanical cell disruption of Escherichia coli for the release of recombinant green fluorescent protein
2012
Nanoglue: An alternative way to display cell-internalizing peptide at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery
2012
Purification of recombinant hepatitis B core antigen from unclarified Escherichia coli feedstock using phage-immobilized expanded bed adsorption chromatography
2012
Delivery of chimeric hepatitis B core particles into liver cells
2012
Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid
2012
Isolation and identification of lactic acid bacteria from fermented red dragon fruit juices
2011
Virus-like particles of Macrobrachium rosenbergii nodavirus produced in bacteria
2011
Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays
2011
Production of long helical capsid of Nipah virus by Pichia pastoris
2010
Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography
2010
Purification of bacteriophage M13 by anion exchange chromatography
2009
Mutagenesis of the Nucleocapsid Protein of Nipah Virus Involved in Capsid Assembly
2009
Display of Hepatitis B Virus PreS1 Peptide on bacteriophage T7 and Its Potential in Gene Delivery into HepG2 Cells
2009
N-terminally His-tagged hepatitis B core antigens: construction, expression, purification and antigenicity
2009
Production of the matrix protein of Nipah virus in Escherichia coli: virus-like particles and possible application in diagnosis
2008
Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.
2007
A phage-displayed single chain variable fragment that interacts with hepatitis B core antigen: library construction, selection and diagnosis
2005
A phage-displayed cyclic peptide that interacts tightly with the immunodominant region of hepatitis B surface antigen
2005
Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7
2003
Selection of high affinity ligands to Hepatitis B core particles from a phage-displayed cyclic peptide library