PROFILE
UPM's Staff Profile
PUAN NUR FADHILAH BINTI KHAIRIL MOKHTAR
PEGAWAI PENYELIDIK
OFFICE OF THE DEPUTY VICE CHANCELLOR (RESEARCH & INNOVATION)
PROFESSIONAL
A Research Officer at Halal Products Research Institute focusing on research on molecular-based detection and profiling method for species identification meant for Halal and food authentication, besides significant interest in food pathogen characterization through next generation sequencing.
Email: nuradhilah@upm.edu.my
Office: +60397691344
Mobile: +60122824834
Email: nuradhilah@upm.edu.my
Office: +60397691344
Mobile: +60122824834
NAME :
(Puan) Nur Fadhilah Binti Khairil Mokhtar
POSITION :
Pegawai Penyelidik
ENTITY :
Office Of The Deputy Vice Chancellor (research & Innovation)
DOWNLOAD CURRICULUM VITAE (PDF)
EDUCATIONAL BACKGROUND
2021
DOCTOR OF PHILOSOPHY (SCIENCE) GENETIC ENGINEERING AND MOLECULAR BIOLOGY
2018
BACHELOR SC. BIOTECHNOLOGY
WORKING EXPERIENCE
2009
Halal Products Research Institute, UPM
2007
Felda Biotechnology Centre
PROJECT
2021
Characterization of antimicrobial peptide from Paenibacilluspolymyxa Kp10 and elucidation of its antimicrobial and antibiofilmmechanistic role against Methicillin-resistant Staphylococcus aureusand Klebsiella penumoniae
2019
Discovery of porcine-specific microRNA marker for halal meatauthentication
2018
Development of novel porcine-specific DNA aptamer for rapidauthentication of porcine meat and its derivatives
2018
Validation and certification for pre-commercialization of LAMPporcine detection kit
2017
DNA extraction and porcine detection by using conventional and realtime PCR for fat and oil
2017
Development of DNA extraction kit for lard-containing flour-basedbakery products
2017
Development of expression vector for high throughput expression ofrecombinant bacteriocin from Pediococcus acidilactici Kp10 inEscherichia coli
2017
Mechanistic study of antimicrobial peptides from locally isolatedlactic acid bacteria against antibiotic-resistant pathogens
2016
Molecular characterization and determination of antiviral propertiesof putative antimicrobial peptides from Pediococcus acidilacticiKp10
2013
Isotopic composition and DNA barcoding for tracing the origin ofhalalal toyyiban foodstuffs
2012
Molecular beacon real time PCR assay targeting chromosomal andmitochondrial-encoded gene for quantification of porcine DNA inhighly processed products
2012
Development of porcine collagen detection technique: bimolecularfluorescence complementation (BiFC) of green fluorescent proteinusing specific binding of protein to porcine collagen
2012
Penyelidikan isu kefatwaan: kuantiti dan stabiliti DNA babi di dalamproduk makanan diproses
2009
Halal verification technique development: TaqMan real-timepolymerase chain reaction (PCR) assay for specific detection of porkand its derivatives
2009
Development of halal food authentication technique: screening andcharacterization of thermostable species-specific protein in porcine
RESEARCH INTEREST
DNA AND RNA-BASED DETECTION METHOD DEVELOPMENT AND VALIDATION FOR HALAL FOOD AUTHENTICATION;ADOPTION OF NEXT GENERATION SEQUENCING IN FOOD PATHOGEN CHARACTERIZATION
CURRENT RESEARCH
2023
METHOD DEVELOPMENT AND VALIDATION OF DIGITAL PCR FOR FOOD AUTHENTICATION
2023
METHOD DEVELOPMENT AND VALIDATION FOR SPECIES PROFILING
2023
DNA EXTRACTION METHOD OPTIMIZATION FROM DIFFICULT SAMPLES
PATENT
2017
‘Method and kit to detect porcine in food or nonfooditems by isothermal amplification technique’. Filing No: PI 2017702029.
2010
‘Porcine Detection and Methods Thereof’,Application No: PI 2010 700044
PUBLICATION
1) Che Man, Y.B*, Shuhaimi, M., Khairil Mokhtar, N.F., Rumaisa, N.,Sazili, A.Q. (2012). Porcine-specific polymerase chain reaction based on mitochondrial D-loop gene for identification of pork in raw meat. International Journal of Food Properties, 15:134-144
2) Yusop, M.H.M., Shuhaimi, M*., Che Man, Y.B., Khairil Mokhtar,N.F. (2012) Detection of raw pork targeting porcine-specific mitochondrial cytochrome b gene by molecular beacon probe real-time polymerase chain reaction. Food Analytical Methods. 5:422-429
3) Mohamad, N.A., El Sheikha, A.F., Mustafa, S*., and Mokhtar, N.F.K. (2013) Comparison of gene nature used in real-time PCR for porcine identification and quantification: A review. Food Research International. 50: 330-338
4) Sarah, S.A., Karsani, S.A., Amin, I., Mokhtar, N.F.K., Sazili, A.Q.(2014) A proteomic based assessment on changes in myofribillar proteins of goat longissimus muscle as affected by heat treatments. The Journal of Animal and Plant Sciences. 24(2): 406-412
5) Mohamad, N.A., Mustafa, S*., El Sheikha, A.F., Mokhtar, N.F.K., Amin, I., Ali, M.E. (2015) Modification of gelatin-DNA interaction for optimized DNA extraction from gelatin and gelatin capsule. Journal of the Science of Food and Agriculture. DOI: 0.1002/jsfa.7482
6) Mokhtar, N.F.K*., Raha, A.R., Amalia, M.H., Mustafa, S. (2016) Collagen binding ability of bacterial isolated from pig and cow small intestine. Sains Malaysiana 45(3): 411–416.
7) Rosman, N.N., Mokhtar, N.F.K., Ali, M.E., Mustafa, S*. (2016) Inhibitory Effect of Chocolate Components Toward Lard Detection in Chocolate Using Real Time PCR. International Journal of Food Properties 19 (11), 2587-2595
8) SA Sarah, SA Karsani, NFK Mokhtar, AQ Sazili, I Amin (2013) Differences in thermostable actin profile of goat meat as observed in two-dimensional gel electrophoresis (2DE). International Food Research Journal 20 (2)
9) El Sheikha, A. F., Mokhtar, N.F.K., Ceesay, A., Dhilia, U.L.,Nurulfiza, M.I., and Shuhaimi, M*. (2017) Authentication technologies using DNA-based approaches for meats and halal meats determination. Food Biotechnology 31(4)281-315
10) Nhari, R.M.H.R., Mokhtar, N.F.K., Hanish, I., Hamid, M*, Rashidi, M.A.A.M., Shahidan, N.M. (2018) Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi. Food Additives and Contaminants Part A. 35(5):807-817
11) Mohamad, N.A., Mustafa, S., Mokhtar, N.F.K*., El Sheikha, A.F., Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules. (2018) Journal of the Science of Food and Agriculture 98(12):4570-4577
12) Tasrip, N. A., Khairil Mokhtar, N. F., Hanapi, U. K., Abdul Manaf, Y. N., Ali, M. E., Cheah, Y. K., Mustafa, S. and Mohd Desa, M. N. (2019) Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat-based food products. International Food Research Journal 26(1)
13) Abd-Gani, S.S., Mustafa, S., Mohd Desa, M.N., Khairil Mokhtar, N.F., Hanapi, U.K., Zakaria, Z. Yahaya, N., Wan Sulaiman, W.M.A. (2018) Detection of porcine adulteration in cosmetic cream formulation via TaqMan Probe Real-time Polymerase Chain Reaction. International Journal of Engineering and Technology 7(4.14) 112-115
14) NF Khairil Mokhtar*, AF El Sheikha, NI Azmi, S Mustafa (2020) Potential authentication of various meat‐based products using simple and efficient DNA extraction method. Journal of the Science of Food and Agriculture 100 (4), 1687-1693
15) RMH Raja Nhari, AN Muhammad Zailani, NF Khairil Mokhtar, I Hanish. (2020) Detection of porcine pepsin in model cheese using polyclonal antibody-based ELISA. Food Additives & Contaminants: Part A, 37(4):561-567
16) Qamar Zia, Mohammad Alawami, Nur Fadhilah Khairil Mokhtar,
Raja Mohd Hafidz Raja Nhari, Irwan Hanish. (2020) Current
analytical methods for porcine identification in meat and meat
products. Food Chemistry 324 (126664)
17) NF Khairil Mokhtar, I Hanish, A Mohd Hashim, A Zulkarnain,
(2020) The discovery of new antilisterial proteins from
Paenibacillus polymyxa Kp10 via genome mining and mass
spectrometry. Frontiers in Microbiology. 11, 960
18) N Sajali, SC Wong, S Abu Bakar, NF Khairil Mokhtar, YN
Manaf, et al. (2021) Analytical approaches of meat authentication in
food. International Journal of Food Science & Technology 56 (4),
1535-1543
19) NI Azizan, NFK Mokhtar*, S Arshad, SN Sharin, N Mohamad, S
Mustafa, et al (2021) Detection of lard adulteration in wheat biscuits
using chemometrics-assisted GCMS and random forest. Food
Analytical Methods 14 (11), 2276-2287
20) NA Tasrip, MN Mohd Desa, NF Khairil Mokhtar, N Sajali, A
Mohd Hashim, et al (2021) Rapid porcine detection in gelatin-based
highly processed products using loop mediated isothermal
amplification. Journal of Food Science and Technology 58 (12),
4504-4513
21) NFK Mokhtar*, AM Hashim, S Abbasiliasi, A Zulkarnain, RMH
Raja Nhari, et al (2021) Physicochemical stability of antilisterial
proteins from P. polymyxa Kp10 as potential food biopreservative.
International Journal of Food Science & Technology 56 (12), 6549-
6558
22) UNM Asri, NFK Mokhtar*, RMHR Nhari, MH Yuswan, AM
Hashim, et al (2021) Mass spectrometry determination of potential
species-specific peptide markers in commercial seasoning cubes.
Journal of Food Composition and Analysis 104, 104193
23) NHZ Baharin, NFK Mokhtar, MNM Desa, B Gopalsamy, NNM
Zaki, et al (2021) The characteristics and roles of antimicrobial
peptides as potential treatment for antibiotic-resistant pathogens: a
review. PeerJ 9, e12193
2) Yusop, M.H.M., Shuhaimi, M*., Che Man, Y.B., Khairil Mokhtar,N.F. (2012) Detection of raw pork targeting porcine-specific mitochondrial cytochrome b gene by molecular beacon probe real-time polymerase chain reaction. Food Analytical Methods. 5:422-429
3) Mohamad, N.A., El Sheikha, A.F., Mustafa, S*., and Mokhtar, N.F.K. (2013) Comparison of gene nature used in real-time PCR for porcine identification and quantification: A review. Food Research International. 50: 330-338
4) Sarah, S.A., Karsani, S.A., Amin, I., Mokhtar, N.F.K., Sazili, A.Q.(2014) A proteomic based assessment on changes in myofribillar proteins of goat longissimus muscle as affected by heat treatments. The Journal of Animal and Plant Sciences. 24(2): 406-412
5) Mohamad, N.A., Mustafa, S*., El Sheikha, A.F., Mokhtar, N.F.K., Amin, I., Ali, M.E. (2015) Modification of gelatin-DNA interaction for optimized DNA extraction from gelatin and gelatin capsule. Journal of the Science of Food and Agriculture. DOI: 0.1002/jsfa.7482
6) Mokhtar, N.F.K*., Raha, A.R., Amalia, M.H., Mustafa, S. (2016) Collagen binding ability of bacterial isolated from pig and cow small intestine. Sains Malaysiana 45(3): 411–416.
7) Rosman, N.N., Mokhtar, N.F.K., Ali, M.E., Mustafa, S*. (2016) Inhibitory Effect of Chocolate Components Toward Lard Detection in Chocolate Using Real Time PCR. International Journal of Food Properties 19 (11), 2587-2595
8) SA Sarah, SA Karsani, NFK Mokhtar, AQ Sazili, I Amin (2013) Differences in thermostable actin profile of goat meat as observed in two-dimensional gel electrophoresis (2DE). International Food Research Journal 20 (2)
9) El Sheikha, A. F., Mokhtar, N.F.K., Ceesay, A., Dhilia, U.L.,Nurulfiza, M.I., and Shuhaimi, M*. (2017) Authentication technologies using DNA-based approaches for meats and halal meats determination. Food Biotechnology 31(4)281-315
10) Nhari, R.M.H.R., Mokhtar, N.F.K., Hanish, I., Hamid, M*, Rashidi, M.A.A.M., Shahidan, N.M. (2018) Monoclonal antibody-based enzyme immunoassay for detection of porcine plasma in fish surimi. Food Additives and Contaminants Part A. 35(5):807-817
11) Mohamad, N.A., Mustafa, S., Mokhtar, N.F.K*., El Sheikha, A.F., Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules. (2018) Journal of the Science of Food and Agriculture 98(12):4570-4577
12) Tasrip, N. A., Khairil Mokhtar, N. F., Hanapi, U. K., Abdul Manaf, Y. N., Ali, M. E., Cheah, Y. K., Mustafa, S. and Mohd Desa, M. N. (2019) Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat-based food products. International Food Research Journal 26(1)
13) Abd-Gani, S.S., Mustafa, S., Mohd Desa, M.N., Khairil Mokhtar, N.F., Hanapi, U.K., Zakaria, Z. Yahaya, N., Wan Sulaiman, W.M.A. (2018) Detection of porcine adulteration in cosmetic cream formulation via TaqMan Probe Real-time Polymerase Chain Reaction. International Journal of Engineering and Technology 7(4.14) 112-115
14) NF Khairil Mokhtar*, AF El Sheikha, NI Azmi, S Mustafa (2020) Potential authentication of various meat‐based products using simple and efficient DNA extraction method. Journal of the Science of Food and Agriculture 100 (4), 1687-1693
15) RMH Raja Nhari, AN Muhammad Zailani, NF Khairil Mokhtar, I Hanish. (2020) Detection of porcine pepsin in model cheese using polyclonal antibody-based ELISA. Food Additives & Contaminants: Part A, 37(4):561-567
16) Qamar Zia, Mohammad Alawami, Nur Fadhilah Khairil Mokhtar,
Raja Mohd Hafidz Raja Nhari, Irwan Hanish. (2020) Current
analytical methods for porcine identification in meat and meat
products. Food Chemistry 324 (126664)
17) NF Khairil Mokhtar, I Hanish, A Mohd Hashim, A Zulkarnain,
(2020) The discovery of new antilisterial proteins from
Paenibacillus polymyxa Kp10 via genome mining and mass
spectrometry. Frontiers in Microbiology. 11, 960
18) N Sajali, SC Wong, S Abu Bakar, NF Khairil Mokhtar, YN
Manaf, et al. (2021) Analytical approaches of meat authentication in
food. International Journal of Food Science & Technology 56 (4),
1535-1543
19) NI Azizan, NFK Mokhtar*, S Arshad, SN Sharin, N Mohamad, S
Mustafa, et al (2021) Detection of lard adulteration in wheat biscuits
using chemometrics-assisted GCMS and random forest. Food
Analytical Methods 14 (11), 2276-2287
20) NA Tasrip, MN Mohd Desa, NF Khairil Mokhtar, N Sajali, A
Mohd Hashim, et al (2021) Rapid porcine detection in gelatin-based
highly processed products using loop mediated isothermal
amplification. Journal of Food Science and Technology 58 (12),
4504-4513
21) NFK Mokhtar*, AM Hashim, S Abbasiliasi, A Zulkarnain, RMH
Raja Nhari, et al (2021) Physicochemical stability of antilisterial
proteins from P. polymyxa Kp10 as potential food biopreservative.
International Journal of Food Science & Technology 56 (12), 6549-
6558
22) UNM Asri, NFK Mokhtar*, RMHR Nhari, MH Yuswan, AM
Hashim, et al (2021) Mass spectrometry determination of potential
species-specific peptide markers in commercial seasoning cubes.
Journal of Food Composition and Analysis 104, 104193
23) NHZ Baharin, NFK Mokhtar, MNM Desa, B Gopalsamy, NNM
Zaki, et al (2021) The characteristics and roles of antimicrobial
peptides as potential treatment for antibiotic-resistant pathogens: a
review. PeerJ 9, e12193